Journal: Journal of Cancer

Article Title: Clinicopathological Significance and Antitumor Effect of MPHOSPH1 in Testicular Germ Cell Tumor

doi: 10.7150/jca.25279

Figure Lengend Snippet: The efficacy of MPHOSPH1 knockdown using small-interfering RNA (siRNA). A . Protein was extracted from the cell lines at 24 h and 48 h post-transfection. Knockdown of MPHOSPH1 was confirmed by Western blotting ( A ). MPHOSPH1 knockdown significantly reduced cell migration ( B ) and cell invasion ( C ) in both NEC8 and NEC14 ( p <0.001 for both). MPHOSPH1 knockdown also significantly inhibited cell proliferation ( D ) and colony formation ( E ) in both of the cell lines ( p <0.001 for both).

Article Snippet: The human embryonal carcinoma cell lines NEC8 and NEC14 were purchased from the Riken Bioresource Center (Tsukuba, Japan) and cultured in RPMI-1640 medium with 10% fetal bovine serum (FBS).

Techniques: Knockdown, Small Interfering RNA, Transfection, Western Blot, Migration

Journal: Oncotarget

Article Title: Angiotensin II type I receptor (AT1R) is an independent prognosticator of esophageal squamous cell carcinoma and promotes cells proliferation via mTOR activation

doi: 10.18632/oncotarget.11567

Figure Lengend Snippet: A. Serum-starved CE81T/VGH, CE48T/VGH, and CE146T/VGH cells treated with or without angiotensin II stimulation were seeded into 96-well plates with 1.0% of FBS. The cells were cultured for 72 hours followed by MTT assay (OD570) to quantitate cell growth. The data were normalized against the OD570 value on control group (0 μM) of each treatment. B. Serum-starved cells were pre-treated with or without various concentrations of irbesartan or losartan or PD123319 for 30 mins; the cells were then stimulated with angiotensin II (10 μM). The cells were cultured for 72 hours followed by MTT assay to quantitate cell growth. C. The mRNA and protein expression profiles of AT1R and AT2R in ESCC cell lines were determined by Q-RT-PCR and Western blotting. D and E. The abilities of cell growth, colony formation, and BrdU incorporation in AT1R-depleted CE81T/VGH cells or siControl group with or without angiotensin II (10 μM) stimulation were assayed. F. The abilities of colony formation and BrdU incorporation in angiotensin II-stimulated CE81T/VGH cells treated with or without irbesartan were assayed.

Article Snippet: The CE81T/VGH and CE48T/VGH ESCC cell lines were obtained from the Bioresource Collection and Research Center (BCRC), and cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum, 2 mmol/L glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin.

Techniques: Cell Culture, MTT Assay, Control, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, BrdU Incorporation Assay

Journal: Oncotarget

Article Title: Angiotensin II type I receptor (AT1R) is an independent prognosticator of esophageal squamous cell carcinoma and promotes cells proliferation via mTOR activation

doi: 10.18632/oncotarget.11567

Figure Lengend Snippet: ( A , left panel) The AKT activation was examined in AT1R-depleted cells with or without angiotensin II stimulation. ( A , right panel) the phosphorylated status of mTOR was determined in ESCC cells stimulated with angiotensin II by Western blotting. B. Serum-starved cells were pre-treated with indicated concentrations of everolimus for 30 mins; the cells were then stimulated with or without angiotensin II. The cells were cultured for 72 hours followed by MTT assay to quantitate cell growth. In addition, the abilities of colony formation and BrdU incorporation in angiotensin II-stimulated CE81T/VGH cells treated with or without everolimus were assayed. C. The protein expression levels of total mTOR, phosphorylated mTOR and AT1R were demonstrated in CE81T/VGH cells transfected with siControl and simTOR. The cell growth abilities of siControl and simTOR stimulated with angiotensin II were measured by MTT assay. D. The protein expression profiles of AT1R, total mTOR, and phosphorylated mTOR were determined in AT1R-depleted CE81T/VGH cells.

Article Snippet: The CE81T/VGH and CE48T/VGH ESCC cell lines were obtained from the Bioresource Collection and Research Center (BCRC), and cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum, 2 mmol/L glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin.

Techniques: Activation Assay, Western Blot, Cell Culture, MTT Assay, BrdU Incorporation Assay, Expressing, Transfection

Journal: Oncotarget

Article Title: Angiotensin II type I receptor (AT1R) is an independent prognosticator of esophageal squamous cell carcinoma and promotes cells proliferation via mTOR activation

doi: 10.18632/oncotarget.11567

Figure Lengend Snippet: A. The incidence of esophageal tumor in mice treated with irbesartan was significantly lower than that in mice treated with vehicle control (57% versus 89%; P = 0.034). B. Gross appearance of esophagus from representative mice treated with irbesartan or vehicle control. The arrows indicate an enlargement of esophageal tumor. C. Hematoxylin and eosin stained (H&E) sections from representative mice treated with vehicle control showed esophageal squamous cell carcinoma with muscle invasion. H&E sections from representative mice treated with irbesartan showed only esophageal dysplasia. Compared to the vehicle control group, immunohistochemistry revealed lower AT1R and p-mTOR expression in the irbesartan group. The magnified figures are shown in the upper right corner. Original magnification ×200. SCC: squamous cell carcinoma.

Article Snippet: The CE81T/VGH and CE48T/VGH ESCC cell lines were obtained from the Bioresource Collection and Research Center (BCRC), and cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum, 2 mmol/L glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin.

Techniques: Control, Staining, Immunohistochemistry, Expressing